Haematoxylin and eosin ( 400 magnification) Open in a separate window Figure 6 Bone marrow

Haematoxylin and eosin ( 400 magnification) Open in a separate window Figure 6 Bone marrow. Reports supplemental_fig3 C Supplemental material for Blastic natural killer cell lymphoma/leukaemia in a cat supplemental_fig3.tif (23M) GUID:?4BEE5C78-89A3-4285-9042-A7D89E92C127 Supplemental material, supplemental_fig3 for Blastic natural killer cell lymphoma/leukaemia in a cat by Miyuki Hirabayashi, James K Chambers, Mei Sugawara, Aki Ohmi, Hajime Tsujimoto, Hiroyuki Nakayama and Kazuyuki Uchida in Journal of Feline Medicine and Surgery Open Reports supplemental_fig4 C Supplemental material for Blastic natural killer cell lymphoma/leukaemia in a cat supplemental_fig4.tif (7.9M) GUID:?84551CCF-D753-42FC-932E-B7DBF1E223B4 Supplemental material, supplemental_fig4 for Blastic natural killer cell lymphoma/leukaemia in a cat by Miyuki Hirabayashi, James K Chambers, Mei Sugawara, Aki Ohmi, Hajime Tsujimoto, Hiroyuki Nakayama and Kazuyuki Uchida in Journal of Feline Medicine and Surgery Open Reports Abstract Case summary A 7-year-old mixed-breed cat presented with subcutaneous oedema and erythema extending from the right axilla to the abdomen. Fine-needle aspiration of the subcutaneous lesion revealed large, atypical, round cells. A clonality analysis for the T-cell receptor-gamma and immunoglobulin heavy chain genes showed no clonal rearrangement. The presumed diagnosis was lymphoma and the cat was treated with prednisolone and L-asparaginase but died 78 days after initial treatment. At necropsy, an oedematous subcutaneous mass in the right axilla, hepatomegaly, splenomegaly and lymphadenopathy of the mediastinum and left axilla were observed. Histopathological examination revealed diffuse infiltration of large atypical round cells in the Voruciclib hydrochloride subcutaneous mass, liver, spleen, lymph nodes and bone marrow. Immunohistochemically, the tumour cells were strongly positive for CD56, and negative for CD3, CD20, CD79a, CD57, granzyme B and perforin. Based on these findings, the cat was diagnosed with blastic natural killer (NK) cell lymphoma/leukaemia. Relevance and novel information Here, we report the pathological and clinical findings of NK cell lymphoma/leukaemia in a cat. The antibody for human CD56, a diagnostic marker for human NK cell neoplasms, showed cross-reactivity with feline CD56 by immunohistochemistry and Western blotting analysis. The antibody could be a useful diagnostic marker for feline NK cell neoplasms. strong class=”kwd-title” Keywords: NK cell lymphoma/leukaemia, CD56 Introduction Natural killer (NK) cell neoplasms are rare both in humans and in animals. In humans and rodents, NK cells have been characterised by the expression of CD56 and the absence of surface CD3 and T-cell receptor (TCR). NK cells arise from the same lymphocyte precursor cell as T cells Voruciclib hydrochloride and B cells. NK progenitor cells express CD34 and CD10, as well as other myeloid and lymphoid progenitor cells. Immature NK cells express CD56 and lack CD34 and CD10 expression, and eventually express CD16 and CD57 during maturation. CD56, CD16 and CD57 are used as markers for diagnosing NK cell neoplasms in humans.1C4 In animals, there have been a few studies on the immunophenotypes and functions of NK cells. 5C7 In dogs and cats, NK cells are negative for surface CD3 by flow cytometry,7C9 but an antibody that specifically labels surface CD3 is not available for immunohistochemistry on formalin-fixed paraffin embedded sections. Therefore, NK cell neoplasms have been diagnosed tentatively in cases of non-T, non-B lymphoma/leukaemia.10 There has been only one case report of blastic NK cell leukaemia in a dog, which was diagnosed based on the detection of CD56 mRNA expression by a reverse transcription PCR analysis.8 Here, we report pathological and clinical findings of NK cell lymphoma/leukaemia in a cat. The immunohistochemical findings of the neoplastic Rabbit Polyclonal to RHOB cells revealed that Voruciclib hydrochloride CD56 can be used as a marker for feline NK cell neoplasm in routine histopathological examinations. Case description A 7-year-old neutered male, mixed-breed cat presented with subcutaneous oedema and erythema of the right axilla extending to the abdomen, and swelling of the right forelimb (Figure 1). Clinical examinations revealed mild fever (39.7C) and a subcutaneous soft mass (3.0 2.0 cm) on the back. A complete blood count revealed anaemia (packed cell volume [PCV] 26%) and thrombocytopenia (96,000 platelets/l) (Table 1). A blood smear examination revealed a small number of large atypical round cells. The cells had small-to-moderate amounts of basophilic cytoplasm and large nuclei with finely diffused chromatin and several nucleoli. The cells rarely contained fine azurophilic granules in the cytoplasm (supplementary Figure 1). Examinations for feline immunodeficiency virus antibodies and feline leukaemia virus antigens were not performed. Thoracic and abdominal radiography and abdominal ultrasonography showed no significant findings. Cytological analysis with fine-needle aspiration from the subcutaneous lesion of the right axilla and the dorsal subcutaneous mass revealed the presence of large atypical round cells. The cells had small-to-moderate amounts of basophilic cytoplasm and irregular nuclear membranes, and were occasionally binucleated (Figure 2). The nucleus had.