The significance level was set at 0

The significance level was set at 0.05 modified with Bonferroni correction for multiple Lep testing. from progressive supranuclear palsy (PSP) and healthy controls (CON). Results Individuals suffering from anti-IgLON5 disease differed from PSP most strikingly in terms of saccade velocity and accuracy, the presence of square wave jerks (SWJ) (anti-IgLON5 0/4 the lateral geniculate nucleus to the striate cortex and extrastriate areas. Further main neural areas involved in smooth pursuit attention movements are the frontal attention field, the supplemental attention field, the pontine nuclei and the cerebellum. In normal subjects the VOR moves the eyes at the same rate but in the opposite direction to the head movement which is definitely accomplished by semicircular canals projecting to the vestibular nuclei and ocular engine nuclei (15). With this small cohort we targeted to explore variations in ocular engine function between individuals with anti-IGLON5 disease and PSP individuals in order to support early analysis of anti-IGLON5 disease. The primary objective was to characterize horizontal and vertical saccadic abnormalities in the above-mentioned diseases in comparison to healthy settings (CON). Further, we focus on differences in clean pursuit attention motions (SPEM), vestibulo-ocular reflex (VOR) function and VOR suppression. Methods We investigated quantitative VOG data of individuals with anti-IgLON5 disease and compared them with those of individuals with progressive supranuclear palsy-parkinsonism (PSP-P) or classic Richardsons syndrome (PSP-RS) and CON with normal ocular Dihydroeponemycin engine function (observe below). VOG data of individuals diagnosed with anti-IgLON5 disease between years 2015 and 2018 and of individuals with PSP, diagnosed between years 2012 and 2018 have Dihydroeponemycin been examined and analyzed retrospectively. Subjects Four individuals suffering from anti-IgLON5 disease and ten individuals diagnosed with PSP (5 PSP-RS, 5 PSP-P) underwent VOG and rotational chair testing. Patients were diagnosed according to the founded 2017 International Parkinsons and Movement Disorder Society (MDS) Criteria for the Analysis of PSP (7). Individuals enrolled before 2017 were revisited and classified according to the recent MDS-PSP criteria for PSP-P and PSP-RS. Analysis of anti-IgLON5 disease was supported by the use of founded criteria for autoimmune-encephalitis and confirmed by anti-IgLON5 antibody detection (1, 14, 16). Auto-antibodies were determined using a well-established in-house routine cell-based-assay as reported earlier (17). No additional antibodies were found in individuals with anti-IgLON5 disease, and no antibodies (including anti-IgLON5 antibodies) were found in individuals with PSP. All individuals underwent VOG at least once, the VOG-recording closest to time of analysis was utilized for analysis. Patients were excluded from VOG if any restrictions were present that would possess limited the individuals cooperation during the exam. The protocol was authorized by the local ethics committee of the Medical University or college of Vienna (EK nr. EK 1773/2016; 1123/2015). Settings Ten sex- and age-matched subjects without history of any neurological disease and with normal neurological exam served as CON. Video-Oculography and Rotational Chair System VOG and a computer-controlled rotational chair system (System 2000, Micromedical Systems, Illinois, USA), were used to assess ocular engine and vestibular function (sampling rate 60 Hz). The individuals head was fixed inside a u-shaped headrest at suboccipital height. Stimuli were applied by a laser projector. Protocol Spontaneous Eye Motions In this task the patients were instructed to fix an imaginary point on Dihydroeponemycin the wall Dihydroeponemycin for 30 mere seconds in total darkness while looking straight ahead and not to move their eyes. Saccades and Square Wave Jerks For horizontal and vertical saccade screening, the target relocated randomly in amplitude methods of 5 – 30 in both directions in the horizontal and vertical aircraft, respectively. Saccades were detected by velocity thresholding algorithms which classified points like a saccade if their velocity was greater than a threshold, and as.