Secondary antibodies conjugated to either Alexa 594 goat against rabbit IgG (H+L) or Alexa 488 fluorophore donkey anti-mouse (both from Molecular Probes, Invitrogen, Carlsbad, CA) in PBS were added, followed by incubation in a dark place at room temperature for 1 h
April 1, 2026
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Secondary antibodies conjugated to either Alexa 594 goat against rabbit IgG (H+L) or Alexa 488 fluorophore donkey anti-mouse (both from…