It has been suggested that low induction heat, low IPTG concentration and solubility tags could improve the solubility of the proteins (13)March 19, 2023
It has been suggested that low induction heat, low IPTG concentration and solubility tags could improve the solubility of the proteins (13). for HTLV-I/-II screening and the strategy presented in this study could be utilized for easy production of this diagnostic protein. codon usage by JAVA codon optimization tool (http://www.jcat.de/) and analyzed by rare codon analysis tool (https://www.genscript.com/tools/rare-codon-analysis). The and restriction sites were inserted into the ends of DNA sequence for cloning purpose. Construction Aloperine of the expression plasmid. The synthetic gene was sub-cloned into the sites of a pGS21a vector to produce pGS21a-RA according to standard DNA manipulation methods. Accuracy of sub-cloning was verified using DNA sequencing. Then, the recombinant plasmid was transformed into chemically qualified BL21 (DE3) cells and the colonies were selected on a LB plate supplemented with 100 g/ml ampicillin. Expression of the recombinant antigen. An BL21 (DE3) colony harboring pGS21a-RA expression vector was produced overnight at 37C in 5 ml of LB medium with 100g/ml ampicillin. This culture was inoculated into a baffled flask that contained 100ml of the LB medium with 100g/ml ampicillin. The culture was incubated at 37C until an OD600 of 0.6 was reached. Expression was induced by adding IPTG (final concentration of 0.4mM) and incubating the cells at 18C for an additional 12h under constant shaking at 250 rpm. The cells were harvested by centrifugation at 5000 rpm for 10min at 4C. The pellets were resuspended in PBS and mixed with 2 sample loading Aloperine buffer heated at 100C for 15 min. The samples Rcan1 were analyzed by SDS-PAGE in 12% polyacrylamide gels. The non-induced controls (as negative controls) were analyzed in parallel. Solubility determination. To check the solubility of His-GST-RA under conditions for expression, the pellets obtained from 50 ml of culture following IPTG induction were Aloperine re-suspended in phosphate buffered saline (PBS) buffer (137 mM NaCl; 2.7 mM KCl; 10 mM Na2HPO4.2H2O; 2 mM KH2PO4, pH 7.4) and subjected to sonication (Bandelin, Germany) on ice. The sonication was carried out at a frequency of 20 kHz for 2 moments, bacteria were pulsed for 6 10 s intervals. After sonication, the cell lysate was centrifuged at 12,000 rpm for 20 min at 4C. The obvious supernatant and pellets were analyzed by SDS-PAGE in 12% polyacrylamide gels. Simple purification of RA. The supernatant was centrifuged at 12,000 rpm for 15 min at 4C and filtered through a 0.22-M filter to remove any insoluble material. Subsequently, the cell lysate supernatant was transferred into a 1 5-cm column packed with 1 ml of Ni+-NTA resin and protein was purified according to the manufacture instructions. A circulation rate of 0.5 ml/min was used in all the chromatographic steps. The protein concentration was decided using the Bradford method (10). The purity of the concentrated RA was analyzed by 12% SDS-PAGE. Western blot analysis of the recombinant RA. The purified RA was separated on 12% SDS-PAGE under reducing conditions and then blotted to nitro-cellulose membrane. The membrane was blocked with blocking buffer (PBS, 0.5% Tween-20, and 5% non-fat dry milk powder) and then incubated overnight with serum containing HTLV antibodies at 4C. After washing, the immunoreactive bands were visualized using horseradish peroxidase (HRP)-conjugated HTLV-I/-II chimeric antigen and 3,3,5,5-tetramethylbenzidine (TMB) substrate answer. Enzyme linked immunosorbent assay (ELISA). The ELISA plate wells were coated with RA (5C30 g/ml) in sodium bicarbonate buffer (50 mM, pH 9.6) and incubated overnight at 4C. The coated wells were washed twice with PBS and then blocked with blocking buffer (1% BSA in PBS with 1% Tween-20) for 2 h at room.