Several of the antigens in the panel were derived from nuclear material, including histones and chromatin, and thus may bear relationship at least with the risk of uveitis, as seen in prior studies(10, 11)

Several of the antigens in the panel were derived from nuclear material, including histones and chromatin, and thus may bear relationship at least with the risk of uveitis, as seen in prior studies(10, 11). cluster 1 were less likely to have attained remission by the follow-up visit. Conclusions Antibodies against ECM and possibly other antigens may identify a sub-group of children with oJIA who will require more aggressive therapy to attain control of the arthritis. Keywords: oligoarticular, juvenile idiopathic arthritis, VU 0364770 antibodies Introduction Oligo-articular juvenile idiopathic arthritis (oJIA) has long been considered to carry the best prognosis among the subtypes of JIA(1). Indeed, a large number of children with this diagnosis respond to conservative therapy consisting of nonsteroidal anti-inflammatory drugs or local corticosteroid injections(2). However, historically, at least 50% of this population has extended to poly-articular disease(3, 4), and even years into the disease course, 40 C 50% continue Rabbit Polyclonal to Akt (phospho-Thr308) to have periods of active disease(5), and one-third develop erosive changes(3). There is no reliable prognostic marker within oJIA. Clinical predictors of disease extension include upper extremity involvement, elevated inflammatory markers, and more than one involved joint at baseline(3, 4). One study showed that early age of onset predicted longer active disease duration(6). There are a few studies that have identified biomarkers that may predict extension. Hunter et al. (2010) reported that decreased synovial fluid (SF) CD4:CD8 ratios and increased synovial fluid expression of multiple inflammatory markers VU 0364770 were associated with an increased likelihood of extension(7). Similarly, Gibson et al. (2009) performed proteomic analysis of SF proteins to identify patterns associated with an extended oligoarticular versus a persistent oligoarticular course(8). With the exception of the ANA, no studies have evaluated more VU 0364770 accessible serum biomarkers, including antibodies. Autontibodies may play a role in oJIA. Although not pathognomonic, a positive ANA is seen in about two-thirds of patient and has long been recognized to be a risk factor for chronic uveitis; there is mixed data on whether it affects the articular prognosis(3, 6, 9). Additional data suggests that antibodies to histones or chromatin may also be associated with an increased risk of uveitis(10, 11). The observation that antibodies offer prognostic information with respect to the uveitis and possibly the overall prognosis associated with oJIA raises the question as to whether as yet unidentified antibodies likewise affect the articular prognosis. To evaluate for this possibility, we screened a population of children with oJIA for the presence of antibodies against an array of autoantigens. Patients and Methods Patients 36 children meeting the International League of Associations for Rheumatology (ILAR) criteria for oJIA were evaluated at Texas Scottish Rite Hospital for Children (TSRHC)(12). 18 children evaluated at TSRHC with a chief complaint of joint pain but found to have a non-inflammatory etiology (e.g. benign hypermobility or amplified pain syndrome) were enrolled as controls. Information on patient demographics, disease duration, routine laboratory studies, joint count, and medication use were recorded. IRB approval from the UT Southwestern Medical Center was obtained in advance of the study, and informed consent was obtained from the legal guardians of all enrolled patients; assent was obtained from children age 10 and older as per local regulations. Antibody profiling 5 C 10 ml of blood was obtained from each subject and stored in aliquots at ?80C. Antigens were purchased from a variety of sources, and the optimal priming concentrations had previously been determined. A complete listing of the antigens used, their sources, and the priming concentrations is available from the authors upon request. The array consists of 105 proteins: 101 auto-antigens and 4 controls. A MicroGrid II microarrayer was used to spot the proteins onto Nitrocellulose-coated 16-pad FAST? slides (Whatman Schleicher & Schleicher BioScience, Keene, NH), with 16 arrays printed per slide. VU 0364770 Antigens were printed in duplicate and randomly distributed on the slides. Details of the hybridization procedure were as described previously(13). Briefly, after pre-treatment with DNAse, serum samples were added to the arrays for 60 minutes and washed, after which Cy3 labeled anti-human IgG (Jackson ImmunoResearch, West Grove PA; 1:500 dilution) was applied. After washing, a Genepix 4000B scanner.